Metagenomics is based on the whole genome shotgun (WGS) sequencing strategy. The total DNA extracted from microbiome was randomly broken into short fragments to construct sequencing library with appropriate size of inserts. By paired end (PE) sequencing mode, the functional and compositional profiles of the whole microbiota are displayed comprehensively, and the key biomarkers can be mined at both the compositional and functional level.
Advantages
1. Directly sequencing the gene fragments in the microbiota samples, maximally reflects the complex and precise ecological functions of the microbiota;
2. Illumina NovaSeq platform, shorter running time, higher sequencing data, and more accurate quantitation of species and functions;
3. Multiple functional annotation databases including KEGG, eggNOG, CAZy, NR, Swiss-Prot, GO, VFDB and CARD to optimize the functional annotation of metagenome profiles;
4. High-resolution compositional profiles at species level and even at the strain level;
5. Multivariate statistical analysis and machine learning methods are introduced to deeply mine the differentiated species and accurately identify the key active biomarkers.
