Gray mold, caused by the fungus Botrytis cinerea, is the
most significant postharvest disease of kiwifruit. In the present study,
iTRAQ with LC-ESI-MS/MS was used to identify the kiwifruit proteins
associated with the response to B. cinerea. A total of 2,487
proteins in kiwifruit were identified. Among them, 292 represented
differentially accumulated proteins (DAPs), with 196 DAPs having
increased, and 96 DAPs having decreased in accumulation in B. cinerea-inoculated
vs. water-inoculated, control kiwifruits. DAPs were associated with
penetration site reorganization, cell wall degradation, MAPK cascades,
ROS signaling, and PR proteins. In order to examine the corresponding
transcriptional levels of the DAPs, RT-qPCR was conducted on a subset of
9 DAPs. In addition, virus-induced gene silencing was used to examine
the role of myosin 10 in kiwifruit, a gene modulating host penetration resistance to fungal infection, in response to B. cinerea infection. The present study provides new insight on the understanding of the interaction between kiwifruit and B. cinerea.
Text link:
https://pubmed.ncbi.nlm.nih.gov/29497428/