The research object of metatranscriptomics is the mRNA of microbiome. The rRNA is removed from total RNA of microbiome, which is reverse transcribed into cDNA, and constructed a suitable length of inserted fragment library. These libraries are sequenced under paired end (PE) sequencing mode to accurately quantify the composition of the active species in the whole microbial community and their expression levels. It is important for identifying the key biomarkers in the microbiota and their biological significance.
Advantages
1. Professional, stable and widely accepted experimental operation for total RNA extraction and rRNA removal;
2. Illumina NovaSeq platform, shorter running time, higher sequencing data, and more accurate quantitation of expression-level species and functions;
3. Multiple functional annotation databases including KEGG, eggNOG, CAZy, NR, Swiss-Prot, GO, VFDB and CARD to optimize the active functional annotation of metatranscriptome profiles;
4. High-resolution active compositional profiles at species level and even at the strain level;
5. Multivariate statistical analysis and machine learning methods are introduced to deeply mine the differentially active species and accurately identify the key active biomarkers.
