CircRNA Sequencing

Abstract

Sheep  colibacillosis is one of the most common bacterial diseases in  large-scale sheep farms. In this study, we orally administered Escherichia coli F17 (E. coli  F17) to lambs to obtain antagonistic and sensitive individuals. We used  RNA-seq to screen for differential circRNAs in the spleens of both  antagonist and sensitive individuals to explore the effect of circRNA on  anti-diarrhoea in sheep. The results showed that 60 differentially  expressed (DE) circRNAs were screened by RNA-seq in the spleen of  antagonistic and sensitive lambs, among which 31 were up-regulated and  29 were down-regulated; q-PCR was used to validate the relative  expression levels of six randomly selected circRNAs in antagonist and  susceptible lambs and found to be consistent with the results of  RNA-seq. Using Miranda analysis of circRNA-miRNA-mRNA interactions, we  found a certain target relationship between 6 circRNAs, 5 miRNAs and 9  mRNAs. The relative expression levels of mRNA in antagonistic and  sensitive lambs were verified by q-PCR and were consistent with the  results of RNA-seq. This study explored the expression profile of  circRNA in the spleen of an antagonistic and susceptible lamb with  diarrhoea and found that differentially expressed circRNAs were helpful  for determining how the lambs resist the pathogenesis of diarrhoea and  provided a scientific basis for lambs to resist diarrhoea.