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Prokaryotic RNA Sequencing

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RNA extraction——RNA quality inspection——Remove rRNA sequence——Fragment RNA sequences——Reverse transcription into cDNA——End repair and add adapter——Fragment sorting and PCR amplification——Sequencing—— Data quality control——Bioinformatics analysis

RNA extraction——RNA quality inspection——Remove rRNA sequence——Fragment RNA sequences——Reverse transcription into cDNA——End repair and add adapter——Fragment sorting and PCR amplification——Sequencing—— Data quality control——Bioinformatics analysis